COVID‐19 vaccination in pediatric solid organ transplant recipients—Current state and future directions – L’Huillier – – Pediatric Transplantation



Adverse events


American Society of Transplantation


Centers for Disease Control and Prevention


COVID-19 Vaccines Global Access


Coronavirus disease-2019


Interferon-gamma release assay


International Pediatric Transplant Association


International Society for Heart and Lung Transplantation


Receptor-binding domain


Severe Acute Respiratory Syndrome Coronavirus 2


Solid organ transplantation




TB skin test


United Kingdom


World Health Organization


years old


Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) emerged at the end of 2019 and continues as a worldwide pandemic more than a year later, with 100 million confirmed cases and over 2 million coronavirus disease-2019 (COVID-19)-related deaths to date according to the World Health Organization (WHO). With limited treatment options, vaccination strategies will be key to prevent severe COVID-19. Thirty COVID-19 vaccines were in various stages of development stages by the summer of 2020.1 Six months on, and a year after the first cases of SARS-CoV-2 were confirmed outside China, several vaccines had sufficient clinical safety and efficacy data to obtain approval by the regulatory health authorities, for example, in the European Union, in the United States of America, and in the United Kingdom (UK); an astonishing feat due to collaboration between governments, industry, and academia. However, so far, no vaccine has been approved for use in individuals <16 years old (yo) as children were not included in the initial clinical trials. Further, immunosuppressed individuals including solid organ transplantation (SOT) recipients were excluded from initial clinical vaccine trials, limiting our understanding of both immunogenicity and safety in this at-risk population. Based on previous data in inactivated vaccines, various transplant societies such as the American Society of Transplantation (AST) and The International Society for Heart and Lung Transplantation (ISHLT) have encouraged SOT recipients to get vaccinated against COVID-19.2-4 Here, members of the International Pediatric Transplant Association (IPTA) review the current general status of COVID-19 vaccines focusing on pediatric-specific issues and provide an outlook on COVID-19 vaccination after pediatric SOT and potential future vaccine research directions. As an international group of pediatric transplant specialists from across different continents, we emphasize the overall importance of a collaborative international effort to tackle this global pandemic for the benefit of our patients across the globe.5 For the purpose of this commentary, pediatric patients refer to individuals <18 years. As additional data emerge in pediatric immunocompromised patients, recommendations may change.


Current vaccines employ a range of strategies and include mRNA-based, subunit with or without adjuvants, replication-deficient viral vectors, inactivated or live virus, and DNA platforms. Broad variability exists across the globe in the assessment, approval, and availability of potential vaccines (Table 1). There are 70 vaccines in human clinical trials and 181 in preclinical development to date.6, 7 Efficacy ranges from 65 to 95% depending on the formulation, dosing, and outcomes assessed. Prevention of symptomatic or serious COVID-19 functioned as the primary outcome in most studies, and prevention of asymptomatic infection remains under investigation. For example, vaccine efficacy in preventing symptomatic COVID-19 in adults after two COVID-19 mRNA-based vaccine doses has proven to be quite robust (95% Pfizer, 95% confidence interval [CI] 90.3–97.6% and 94.1% Moderna, 95%CI 89–96.8).8, 9 The replication-deficient adenoviral vector vaccine from Johnson & Johnson was 66% effective in prevention moderate to severe disease.10 Initial studies have neither included children nor individuals receiving immunosuppression (including SOT recipients), leaving pending questions on immunogenicity, efficacy, and safety in these populations. The landscape of COVID-19 vaccines trials that include children is currently less robust, despite requests by pediatric societies and parents call for timely inclusion of children.11, 12

COVID-19 vaccines platforms, efficacy and approval status as of March 15, 2021







95% in 43 548 participants

USA, Canada, Mexico, UK, Israel, Bahrain, Kuwait, Saudi Arabia, Chile, Panama, Ecuador, Costa Rica, E.U., Norway


94.5% in 30 400 participants


Replication defective adenovirus vector


70% in 11 636 participants

Brazil, South Africa, UK

Johnson & Johnson10

66% overall for moderate to severe disease in 44 325 participants


Gamaleya Research Institute60

91.6% in 19 866 participants

Russia, Hungary


Under Investigation


Protein Subunit (with or without adjuvant)


Sanofi-GSK; Clover-GSK-Dynavax Vector Institute; Medicago; Anhui Zhifei Longcom and the Chinese Academy of Medical Sciences (CAMS)

Under Investigation

Vector Institute: Russia

Inactivated Virus


Wuhan Institute

Beijing Institute


Institute of Medical Biology at CAMS

Bharat Biotech and Indian Council of Medical Research and the National Institute of Virology

Under investigation

Preliminary press release: Sinopharm Beijing 86% in 31,000 participants (press release)

Sinopharm: China, UAE, Bahrain



Inequities are even more apparent in many countries such as in South America, where social inequalities as well as diverse and deficient healthcare systems have created a new epicenter for the pandemic.5 The arrival of COVID-19 vaccines brought hope in many countries, with Argentina being the first country to initiate vaccination in South America. So far, South-American countries have been supplied with various vaccines,13 but vaccination campaigns have been restricted to adults. Several South-American vaccines, such as Cuba’s Soberana 2, are currently being evaluated clinically. Thus far, the cumulative vaccine rate is below 4% (0.1–3.8%) of the population, except for Chile, which reports 17.5%.13 Some countries are still awaiting vaccine receipt through the COVID-19 Vaccines Global Access (COVAX) Facility to start vaccination campaigns.

At the writing of this manuscript, one COVID-19 mRNA-based vaccine is approved for individuals 16–17 years and there are approximately ten clinical studies evaluating COVID-19 vaccination in children registered in (accessed 2/25/2021) (Table 2). Pfizer has completed enrolment of 2000 children 12–15 yo, with plans for age de-escalation enrollment down to 5 yo.14 Both safety and immunogenicity data are expected in Q2 to Q3 of 2021. Similarly, Moderna continues enrolling individuals 12–17 yo,15 and the Oxford-AstraZeneca trial has started enrolling children down to 6 yo in the UK.16 Additional studies are under consideration including children as young as 6 months old, but these are in the planning stages. Pediatric immunocompromised patients and SOT recipients were not included in these studies.

Registered clinical studies evaluating COVID-19 vaccination in children as of March 15, 2021


Ages (y)





2000 (enrollment completed)














Bharat Biotech and Indian Council of Medical Research and the National Institute of Virology





So far, only the Pfizer vaccine is approved in patients <18 yo, with a lower age limit of 16 yo. In the large-scale phase 3 placebo-controlled trial, adverse events (AEs) in vaccinees were mostly mild to moderate, with serious AEs only rarely reported.8 Local AEs mainly consisted of pain at injection site, whereas systemic AEs mainly consisted of fatigue, headache, and muscle pain, with respective rates of 78–83%, 47–59%, 42–52%, and 21–37% in participants 16–55 yo.8 In general, local and systemic AEs were more frequent in patients <55 yo than in older patients.8 Regarding anaphylaxis, early reports suggested an incidence of 1/100 000 doses; however, with expanded administration, it appears to be similar to other vaccinations with 2–3 events per 1 000 000 doses.17, 18

Although SOT recipients have been excluded from recently published COVID-19 vaccine trials, very early data indicate that mRNA-based COVID-19 vaccines are safe in adult SOT recipients.19 This agrees with the generally similar safety profile of inactivated vaccines in immunocompromised and immunocompetent individuals.20 As with any recently marketed vaccine, concerns about vaccine-induced allograft rejection or induction of donor-specific antibodies arise although no currently marketed vaccine has been clearly associated with allograft rejection.21, 22 Indeed, among 187 SOT patients who received the mRNA-based COVID-19 vaccine, none developed acute cellular rejection.19 Altogether, the overall safety profile as well as the potential associations with allograft rejection will have to be carefully evaluated in SOT recipients receiving COVID-19 vaccines.

Based on experience with other inactivated vaccines, mRNA-based vaccines are anticipated to be as safe in children as in individuals ≥16-18 yo.


Based on experience with other inactivated vaccines, the mRNA-based vaccines are expected to be as immunogenic in children as in individuals ≥16 yo. COVID-19 vaccines have been successful at both eliciting a robust SARS-CoV-2-specific immune response and preventing overall and severe COVID-19,8, 9, 23, 24 but data on healthy children, pediatric SOT recipients, or otherwise immunocompromised children are lacking.25 Importantly, transplanted children demonstrate decreased immunogenicity to various vaccines compared with healthy children.26 Therefore, it is very likely that transplanted children will have diminished immune responses to COVID-19 vaccines, although the extent of this impairment is currently unknown. Impaired antibody development following confirmed COVID-19 illness in one study of adult SOT recipients highlights the concern that SOT-associated COVID-19 vaccine immunogenicity impairment will be a challenge.27

Determining correlates of protection after both acute COVID-19 or vaccination and threshold values for these correlates will be useful for reliably evaluating COVID-19 vaccine immunogenicity in future studies of transplanted children.28 It will also facilitate the design and completion of vaccine clinical trials in pediatric SOT recipients given the challenges of enrolling sufficient participants to perform efficacy trial(s). In addition, initial immunobridging data from adult SOT cohorts may provide additional context for pediatric recommendations.

With the high concentrations of neutralizing antibodies generated by COVID-19 vaccines in non-immunocompromised adults, there may still be sufficient immunogenicity in pediatric SOT recipients even accounting for immunosuppression and chronic-disease induced immune impairment. Given the development of SARS-CoV-2 variants, eliciting high-level, functionally neutralizing antibody responses to COVID-19 vaccines in pediatric SOT recipients will likely be critical to enable these children to stay ahead of the viral evolution game.


SARS-CoV-2 generally causes a milder disease in children, and COVID-19-associated morbidity and mortality are lower in children compared with adults. However, severe disease, including respiratory failure or shock during acute infection and the post-infectious, immune-mediated multi-system inflammatory syndrome in children (MIS-C) associated with SARS-CoV-2 underscore that COVID-19 and its sequelae can also be severe in children.29-32 Children account for approximately 13% of all COVID-19 cases in the United States, with an overall rate of 4,030 cases/100,000 children, and contribute to 1.2–3% of all hospitalizations.33 As of February 2021, cumulative COVID-19-related mortality in children surpassed the record influenza-related deaths during the 2019–20 season.33-35 Furthermore, regardless of disease severity, children are susceptible to SARS-CoV-2 and likely contribute to virus transmission under certain circumstances. Thus, preventing SARS-CoV-2 infection could prevent severe disease and post-infectious complications, potentially reducing hospitalizations and providing some relief for already overburdened healthcare systems.

Given concerns for impaired immunogenicity post-transplant, provision of COVID-19 vaccine to pediatric SOT candidates would be ideal when feasible. Ideally, COVID-19 vaccination should be completed at least 2–4 weeks before the projected start of any immunosuppression or SOT, as clinically feasible. When available, pediatric SOT candidates or recipients are encouraged to participate in COVID-19 vaccine clinical trials. In addition, cocooning measures should be encouraged, including offering household contacts of pediatric SOT candidates and recipients COVID-19 vaccination if available.

While attention has focused on SARS-CoV-2, pediatric SOT patients are vulnerable to other infections as well. The COVID-19 pandemic has led to disruptions in children’s health maintenance and significant declines in routine childhood vaccinations globally.36 School closures have also impacted vaccine delivery, particularly in countries where vaccines are delivered at school. It remains imperative that SOT candidates receive routine vaccines pre-SOT. Currently, mRNA-based COVID-19 vaccines are not recommended to be co-administered with other vaccines.22 Instead, a 14-day minimum interval between COVID-19 vaccine and any other vaccine is suggested.37 If, however, there is a tight timeline and benefits of vaccination outweigh potential risks of vaccine co-administration, a shorter dosing interval may be justified. Providers should consider combination vaccines, accelerated vaccine schedules, and vaccine pre-planning, including possible prioritization of vaccines (for example, measles-mumps-rubella and varicella) to optimize pre-SOT vaccination strategies. If the candidate is ineligible or unable to receive COVID-19 vaccine before SOT, reassessment of eligibility for COVID-19 vaccination at 1–6 months after SOT is advised,3, 38 with timing depending on induction and maintenance immunosuppression regimens.

The pre-SOT evaluation may also include assessment and testing for tuberculosis (TB) infection. COVID-19 vaccines are not expected to affect the intradermal tuberculin skin test (TST) or TB interferon-gamma release assays (IGRA). However, there are insufficient data regarding whether COVID-19 vaccination could affect the TST and IGRA results in the 4 weeks post-vaccination. Per current Centers for Disease Control and Prevention (CDC) recommendations, if the COVID-19 mRNA-based vaccine has been given, deferring the TST or IGRA testing for ≥4 weeks after completion of the 2-dose COVID-19 vaccine is preferred.39 Prioritization of TB testing in candidates should be based on individual exposures, risk factors, and time to projected SOT and weighed against the importance of receiving COVID-19 vaccine.39

If the SOT candidate has a history of current or prior SARS-CoV-2 infection, then COVID-19 vaccination should be offered after full recovery from COVID-19 and isolation is discontinued.3, 37, 38 If, however, the SOT candidate received adjunctive specific COVID-19 therapies such as convalescent plasma or monoclonal antibodies, then COVID-19 vaccination should be deferred for at least 90 days since last antibody dose to avoid potential effect on vaccine-induced immunogenicity. Individuals receiving non-COVID-19-specific antibody therapies (subcutaneous or intravenous immunoglobulin) may proceed with COVID-19 vaccine when eligible and available, without further delay. SOT candidates who have been exposed to SARS-CoV-2 but have not developed symptomatic infection may seek vaccination after quarantine completion. As current recommendations state that COVID-19 vaccines should not be withheld from pregnant individuals, emerging data demonstrate transplacental antibody transfer, suggesting that young SOT candidates <6 months of age may be protected if their mothers suffered from COVID-19 or received COVID-19 vaccination during pregnancy, though extent and duration of protection are unknown.40, 41

Currently, the extent and duration of vaccine-induced immunity are unknown. At this time, a booster COVID-19 vaccine is not recommended after the initial 2-dose primary mRNA-based vaccination. Nor is re-vaccination currently recommended after immune reconstitution in individuals who received one or both doses of mRNA-based COVID-19 vaccine while receiving immunosuppression. Lastly, since a serological correlate of immune protection has not been confirmed, no recommendations exist for antibody testing after COVID-19 vaccination at this time outside of a clinical study. Regardless of vaccine status and until additional data emerge, SOT candidates and recipients should continue to follow the well-known COVID-19 preventative measures during the pandemic.


In the near future, studies in pediatric candidates and SOT recipients are needed to guide preventive strategies for these vulnerable populations. As children receive many immunizations in early life, data on the immunologic impact of COVID-19 vaccines, including de-novo antibody formation post-transplant or implication of crossmatch results pre-transplant, as well as safety of COVID-19 vaccine concurrent with other vaccines deserve a high priority. Specific safety and efficacy data on children before and after transplant are also of great importance. If immunogenicity is inferior compared with healthy children, studies evaluating possible booster doses would be beneficial.


RNA viruses like SARS-CoV-2 predictably experience mutations on a regular basis.42 Given the widespread presence of SARS-CoV-2 with more than 100 million people infected worldwide, the development of clinically significant virus variants potentially containing multiple sites of mutation was inevitable. Depending upon where on the viral genome the mutations occur, their presence may lead to enhanced infectivity, enhanced virulence, and possibly the ability to escape pre-existing immunity derived from prior infection or vaccine.43 While awareness of the presence of these variants has been increasingly recognized recently, it is worth noting that the emergence of the D614G variant identified in early 2020 was thought to account for a substantial increase in spread through increased replication efficiency and transmissibility.44

To date, three major SARS-CoV-2 variants have drawn attention. In August 2020, the so-called UK variant, also known as B.1.1.7 (N501Y), was initially recognized and has been associated with increased transmissibility.45 While there has been speculation of increased virulence, this has not been confirmed at this time.46 Moreover, this variant does not appear at present to “escape” antibody-mediated immunity from prior COVID-19 infection or immunization.47 Immune sera from persons previously vaccinated with mRNA-based COVID-19 vaccine maintained neutralizing titers against the B.1.1.7 lineage.48 In addition, although B.1.1.7 (as of January 25, 2021) was associated with either imported cases or community spread in a total of 70 countries across all 6 WHO regions,49 evidence at this time has not been confirmed to result in worse outcomes or lack of protection from COVID-19 vaccines that are nearing or already approval.

Of greater concern at present is the South African variant, also known as B.1.351 (N501Y.V2). This variant contains many more mutations than D614G or B.1.1.7, including those that involve the receptor-binding domain (RBD).44 While more recently recognized, its presence appears associated with a notable increase in recognized cases. Importantly, this variant does not appear to impact disease severity. However, there is evidence that B.1.351 evades, at least partly, antibody responses to prior SARS-CoV-2 infection and/or immunization. Evaluation of sera containing antibody against SARS-CoV-2 obtained from patients who had received one of the two mRNA-based vaccines demonstrated a reduction in neutralization of B.1.351 in vitro.50, 51 Additionally, data from clinical trial of the Novavax protein sub-unit COVID-19 vaccine in South Africa found a reduced (~50%) efficacy compared with the nearly 90% efficacy for patients in their clinical trial enrolled in the UK.52 Moreover, therapeutic anti-spike protein monoclonal antibodies demonstrate absent neutralization activity against B.1.351.53 These early findings raise significant concerns about the potential for this and future SARS-CoV-2 variants to initiate an immunologic arms race between vaccine development and variant selection.

Finally, the Brazilian variant, also known as P.1 or B.1.1.26, which has a mutation pattern similar to B.1.351 is the most recent variant to draw attention, the first identified case in the USA occurred in late January 2021. While less is known about this variant, it is thought to be closely related to B.1.351 and will likely share its properties.54

With continued global SARS-CoV-2 circulation in association with high rates of infection, additional variants will continue to emerge, evolve, and spread.55, 56 Of major concern is the ongoing potential for the development of escape mutations against immunity derived from prior infection with older strains or from vaccine. In addition, immunocompromised hosts, potentially including SOT recipients, may serve as source for development of SARS-CoV-2 variants.57 Areas of uncertainty include the relative contribution of T-cell responses to conserved peptide epitopes amongst SARS-CoV-2 variants and whether these conserved responses could mitigate the loss of antibody responsiveness described above.58 Increased vigilance and use of molecular sequencing to fully characterize these mutations combined with the ability of newer vaccine platforms to rapidly generate updated vaccines for use as boosters for those previously vaccinated or in combination with the initial vaccines for those not yet vaccinated, does provide hope to minimize the impact of these variants. In the meantime, prevention strategies are the best way to limit the spread of these variants and to slow the emergence of new ones.


So far, data are still lacking about safety, efficacy, and immunogenicity of COVID-19 vaccine in pediatric SOT candidates and recipients. However, excellent efficacy data, associated with robust vaccine-associated immune responses and a good vaccine safety profile in healthy adults, are promising signals that the currently available vaccines will be safe and effective in the pediatric transplant population. Pediatric- and transplant-specific vaccine trials are rapidly needed to confirm the findings generated in the general population.


Paragraphs were divided between authors, with at least two authors contributing to one paragraph. Each author group performed at least one nonsystematic review of the available literature and drafted the paragraph(s) accordingly. All authors critically reviewed the final version of the manuscript.

No original data in this manuscript.


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